Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line
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منابع مشابه
The Activity of Sirtuin 1 in MCF-7 Breast Cancer Cell Line: The Effects of Visfatin
Background & Objectives: Breast cancer is the most common cancer and the second leading cause of cancer deaths among women. Obesity, hormones, and growth factors are the risk factors for this kind of cancer. One of the changes observed in patients suffering from breast cancer is the elevated Visfatin or nicotinamide phosphoribosyl transferase (NAMPT) in their tumor tissues and blood. The increa...
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Fragrant species of the genus Salvia have been attributed many medicinal properties, which include anticancer activity. In the present study, cytotoxic properties of total methanol extract of Salvia chloroleuca Rech. f. & Aellen and its fractions were investigated on MCF-7, a breast carcinoma cell line. Malignant and non-malignant cells were cultured in RPMI medium and incubated with different ...
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Accurate normalization of gene expression data is an absolute prerequisite to obtain reliable results in qPCR analysis. Oenanthe javanica, an aquatic perennial herb, belongs to the Oenanthe genus in Apiaceae family, with known medicinal properties. In the current study, O. javanica was subjected to hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid) and abiotic st...
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ژورنال
عنوان ژورنال: BMC Molecular and Cell Biology
سال: 2020
ISSN: 2661-8850
DOI: 10.1186/s12860-020-00313-x